1. Plate. inactivated preparation of M. pneumoniae strain FN. 2. Conjugate. Conjugated (horseradish peroxidase) goat anti-human IgM (μchain specific). 3. Positive Control (Human Serum). 4. Calibrator (Human Serum). 5. Negative Control (Human Serum). 6. Sample diluent. Tween-20, bovine serum albumin, phosphate-buffered-saline, and goat anti-human IgG (γ-chain specific), (pH 7.2 ± 0.2). 7. TMB: 3,3’,5,5’-tetramethylbenzidine (TMB). 8. Stop solution: 1M H2SO4 0.7M HCl. 9. Wash buffer concentrate (10X): 10X concentrated phosphate-buffered-saline and Tween-20 solution.

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